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Structure of an Escherichia coli N-acetyl-d-neuraminic acid lyase mutant, E192N, in complex with pyruvate at 1.45 Å resolution The structure of a mutant variant of Escherichia coli N-acetyl-d-neuraminic acid lyase (NAL), E192N, in complex with pyruvate has been determined in a new crystal form. It crystallized in space group P212121, with unit-cell parameters a = 78.3, b = 108.5, c = 148.3 Å. Pyruvate has been trapped in the active site as a Schiff base with the catalytic lysine (Lys165) without the need for reduction. Unlike the previously published crystallization conditions for the wild-type enzyme, in which a mother-liquor-derived sulfate ion is strongly bound in the catalytic pocket, the low-salt conditions described here will facilitate the determination of further E. coli NAL structures in complex with other active-site ligands. (Source: Acta Crystallographica Section F) Med WormSmoking linked to chronic back pain Smokers can add another health concern to their list as research shows daily tobacco use can lead to a bad back.A major Canadian study has identified a higher rate of chronic lower back pain among peo... Big News NetworkEmployment, gender, and smoking cessation outcomes in low-income smokers using nicotine replacement therapymdlinx.com Attention Problems Among Video Gamers The epidemic of attention deficit problems has led experts to review activities that appear to be related to the disorder. Excessive couch-behavior -- watching television and playing video games for an extended period -- has often been viewed as ... ... PsychcentralSeizure Meds May Up Risk of Mental Disorders Professionals acknowledge that a history of seizures during infancy increases the risk for development of schizophrenia later in life. A new study among animal models reveals antiepilectic drug treatments (AED) administered when the brain ... ... PsychcentralSmoking linked to chronic lower back pain A major Canadian study has identified a higher rate of chronic lower back pain among people who smoke every day, particularly the young. Professor Michael Cousins said the research suggested their smo... Big News NetworkDrop the Soda Habit and Feel Better Dropping your habit of drinking sugary, caffeinated beverages like Coke and Pepsi could make you feel better, research suggests. Cross-national epidemiologic studies have identified a possible link between sugar intake and rates of major depressive disorders. In addition, when researchers took a look at the diets of people with depression, they found that their sugar consumption was much higher than the general population. It wasn't just sugar that seemed to be a culprit, however. One small cohort trial found that when people eliminated sugar and caffeine from their diet those with unexplained depression experienced improved moods within a week. When caffeine and sugar were reintroduced into their diet, their symptoms returned; but, placebo did not produce the same effect. Giving further credence to the caffeine-depression link, another study found that regular consumption of high levels of caffeine (>750 mg daily) appears to be associated with depression. While it's not for certai AboutTeens With Depression, Other Mental Disorders May Be at Risk for Internet Addiction According to Taiwanese researchers, teens with various mental disorders, such as depression and ADHD (attention deficit hyperactivity disorder), may be more at risk for becoming addicted to using the Internet. The researchers asked 2,293 seventh graders to fill out questionnaires regarding whether they had ADHD, depression, social phobia or abnormal feelings of hostility. Six to 24 months later, they were asked about their Internet usage, such as how many hours they spent on the Internet and what types of sites they visited. The researchers found that boys and girls with ADHD or hostility were more likely to be addicted to the Internet than those without those conditions. Social phobia and depression was associated with Internet addiction in girls, but not boys. At this time, there is no universally agreed upon definition for Internet addiction, but it is generally considered to be excessive use which negatively impacts grades, relationships or the person's emotional state. In the st About |
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9th of September 2009 - Interview of Feedzilla's founder at the IFA in Berlin. [More]
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